![]() ![]() This association was not detected when the effect of HLA-DQB1*06:02 was considered, suggesting that the hypomethylation was possibly derived from HLA-DQB1*06:02. We then performed an association analysis, and found that several CpG sites in the HLA class II region of the patients were significantly hypomethylated in CD4 + and CD8 + T cells. We confirmed that 90.3% of the probes after general filtering in the HLA region do not include frequent SNPs, and are thus suitable for analysis, particularly in Japanese subjects. The criteria were based on a previous study reporting that the presence of frequent SNPs, especially on the 3′ side of the probe, makes the probe unreliable. As the large number of SNPs in the HLA region might interfere with the affinity of the array probes, we conducted a comprehensive assessment of the reliability of each probe. We analyzed array-based DNA methylation and gene expression data for the HLA region in CD4 + and CD8 + T cells that were separated from the peripheral blood mononuclear cells of Japanese subjects (NT1, N = 42 control, N = 42). Although NT1 showed a strong association with human leukocyte antigen ( HLA) -DQB1*06:02, the responsible antigens remain unidentified. ![]() Narcolepsy type 1 (NT1) is caused by a loss of hypothalamic orexin-producing cells, and autoreactive CD4 + and CD8 + T cells have been suggested to play a role in the autoimmune mechanism.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |